Amin Bigham-Sadegh; Saeid Lotfi; Amad Oryan; Iman Hafar
Abstract
Background: Facilitating the fracture healing process is important to increase the fracture healing speed and to decrease the time period till union. This study aimed to evaluate the effects of blood clot and doxycycline on bone healing process.Methods: Twenty mature male New Zealand white rabbits were ...
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Background: Facilitating the fracture healing process is important to increase the fracture healing speed and to decrease the time period till union. This study aimed to evaluate the effects of blood clot and doxycycline on bone healing process.Methods: Twenty mature male New Zealand white rabbits were used, in this study. A defect was created in the middle part of the radius. The rabbits were randomly distributed into 4 groups and the gaps were filled with the graft materials: blood clot, doxycycline, combination of blood clot and doxycycline and control group in which the defect was left empty. Radiographs of operated limbs were taken on 14th, 28th, 42nd and 56th postoperative days. Histologic samples were taken on the 56th day post surgery.Results: On radiographic evaluation significant difference between the groups was not observed (p>0.05). On histopathological evaluation, blood clot and doxycycline groups were superior to control group (P<0.05), also combination of blood clot and doxycycline group was superior to other ones (P<0.05). There was no evidence of graft rejection in any group.Conclusion: This study demonstrated that the combination of blood clot and doxycycline has a better function in bone healing process than other groups.
Siavash Sharifi; Amin Bigham-Sadegh; Ahmad Oryan; Yasamin Alavi
Abstract
AbstractBackground: Nowadays, bone grafts are used in both veterinary and human orthopaedics to stimulate fracture healing and to accelerate the restoration of bone defects. Autografts are still a high standard for comparing different bone-building stimuli. Autografts not only include healing stimulants ...
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AbstractBackground: Nowadays, bone grafts are used in both veterinary and human orthopaedics to stimulate fracture healing and to accelerate the restoration of bone defects. Autografts are still a high standard for comparing different bone-building stimuli. Autografts not only include healing stimulants but also contains cells that do not stimulate immune responses and do not transmit infectious diseases. However, bone grafting is not without side effects such as pain, infection, fracture, blood loss and increased surgical stages, besides the amount of bone removed is limited.Tarantula cubensis venom has an anti-inflammatory and re-epithelialization effect in bovine wound healing on the 14th day; it also plays a role in infection prevention since Theranekron can alter the process of inflammation. This study aims to examine the effect of hydroalcoholic extract of tarantula cubensis on the distal radius fracture healing in rabbitsMethods: This study was conducted on 20 male rabbits, in four study groups of five rabbits. A bone se was extracted from the distal radius. The first group was administrated with one microgram per kilogram of Theranekron injection at the site of the bone defection. The second and third (or negative control) groups were administrated with normal saline injection and no substance (no intervention), respectively. Lastly, the fourth group (or autograft group), the extracted bone segment was returned in its place, and the skin and muscles were sutured. Lateral view radiographs images were taken from rabbits on days of 14, 28, 42 and 56 after surgery.On the eighth week, a bone biopsy was performed to evaluate histopathology. Radiographs and biopsies were statistically examined for fusion rate, ossification activity and remodelling.Results and Conclusion: Both Theranekron and autograft group had similar behaviour and outperformed the normal saline and negative control group; therefore it is concluded that hydroalcoholic extract of tarantula cubensis was effective in distal radius fracture healing.
Amin Bigham-Sadegh; Siavash Sharifi; Iraj Karimi; Ahmad Oryan; Mina Maleki
Abstract
Background: Orthopedic surgeons are trying to find best substitutes for bone grafting in human and veterinary medicine. Bone autografts are still as a golden standard in comparison with other bone grafts. Diced ear cartilage from dog and rabbit have been used on bone healing previously, in the ...
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Background: Orthopedic surgeons are trying to find best substitutes for bone grafting in human and veterinary medicine. Bone autografts are still as a golden standard in comparison with other bone grafts. Diced ear cartilage from dog and rabbit have been used on bone healing previously, in the present study effects of rabbit and bovine rib cartilages will evaluated in bone healing of rabbit bone defect model.
Method: In this study, 20 adult rabbits weighing approximately 2 kg were used. The rabbits were randomly divided into 4 groups. The bone segment was removed from the mid radial bone. In the first group (N = 5) in the gap segement of bovine rib cartilage was implanted. In the second group (N = 5) in the gap, segement of rabbit rib cartilage was implanted. In the third group (empty control group), (N = 5) the defect was left without implantation. Finally, the fourth group (autograft Group), (N = 5) the defect was filled with a same harvested bone. The skin and muscles were sutured routinely. X-rays were taken on 14th , 28th , 42nd and 56th postoperative days.
Result and conclusion: After 8 weeks bone samples were taken from healed area for for histopathological evaluation. The results of our study indicate allogenic and xenogenic cartilage acted almost like autograft groups and were better than empty group.
Iraj Karimi, PhD; Amin Bigham-Sadegh, PhD; Ahmad Oryan, PhD; Zohre Rahmani, DVM
Abstract
Background: Rupture of tendons is a common injury. The outcome of surgical repair of injured tendon is often unsatisfactory. At best, the restored tendon is about half of its initial mechanical properties. In this study the effect of zenologous and autologous platelet gel were compared in rabbit ...
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Background: Rupture of tendons is a common injury. The outcome of surgical repair of injured tendon is often unsatisfactory. At best, the restored tendon is about half of its initial mechanical properties. In this study the effect of zenologous and autologous platelet gel were compared in rabbit model.
Methods: 45 rabbits in the age range of 7 months to 1 year old were divided into autologous, zenologous and control groups. Superficial digital flexor tendon was cut transversely and then sutured with Mayer stitch pattern. Then 5cc of either aotologous or zenologous platelet gel was injected to the incision area. The control group had no material injection. On 7th, 14th, and 28th post-operative days, five rabbits of each group were euthanized and tendons were harvested for histopathological and biomechanical evaluations. The results were analyzed statistically.
Results: Biomechanical factors were significantly superior in the autologous and zenologous groups than the control group. In histopathological examination the autologous groups showed a significant difference in fibroblast maturation in all the tested weeks. The collagen fiber alignment at 7th postoperative day and collagen accumulation on 7th and 28th postoperative days were superior in autologous compared with zenologous and control groups.
Conclusions: Utilization of zenologous platelet gel has a positive effect on tendon healing, but not as good as autologous platelet gel.
